Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes

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Permeability characteristics and osmotic sensitivity of rhesus monkey (Macaca mulatta) oocytes.

BACKGROUND Permeability characteristics and sensitivity to osmotic shock are principal parameters that are important to derive procedures for the successful cryopreservation of mammalian oocytes. METHODS AND RESULTS The osmotically inactive volume of rhesus monkey oocytes was determined by measuring their volumes in the presence of hypertonic solutions of sucrose from 0.2 to 1.5 mol/l, compar...

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Osmotic tolerance and membrane permeability characteristics of rhesus monkey (Macaca mulatta) spermatozoa.

Biophysical characteristics of the plasma membrane, such as osmotic sensitivity and water and cryoprotectant permeability are important determinants of the function of spermatozoa after cryopreservation. A series of experiments was conducted with rhesus macaque spermatozoa at 23 degrees C to determine their: (1) cell volume and osmotically inactive fraction of the cell volume; (2) permeability ...

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Osmotic tolerance limits and properties of rhesus monkey (Macaca mulatta) spermatozoa.

Fundamental cryobiological characteristics of rhesus spermatozoa must be determined for successful cryopreservation techniques to be established. The main objectives of the present study were to determine the osmotic behavior and osmotic tolerance limits of rhesus macaque spermatozoa. Cell volume changes over anisotonic conditions were assessed using an electronic particle counter and sperm mot...

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Histochemical characteristics of the masseter and temporalis muscles of the rhesus monkey (Macaca mulatta).

The histochemical characteristics, cross-sectional area and capillary of the skeletal muscle fibers of the anterior and posterior regions of the superficial masseter and the temporalis muscles are described for juvenile and adult rhesus monkeys of both sexes. Slow twitch fatigue resistant (S), fast twitch fatigue resistant (FR) and fast twitch fatigable (FF) fibers were found in varying proport...

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ژورنال

عنوان ژورنال: Human Reproduction

سال: 2002

ISSN: 0268-1161,1460-2350

DOI: 10.1093/humrep/17.7.1875